Supplementary Materials (Right-Click and Choose 'Save As' to Download Files)


Table S1. Interactions of kinases, phosphatases and associated subunits [excel][pdf]
Table S2. Sequence coverage for epitope tagged bait proteins [excel][pdf]
Table S3. Biological and technical repeats for Swe1 kinase [excel][pdf]
Table S4. Biological and technical repeats for Mps1 kinase [excel][pdf]
Table S5. Effect of bait induction level on spectral count coverage of interaction partners [excel][pdf]
Table S6. List of interactions tested by affinity capture-immunoblot [excel][pdf]
Table S7. Mps1 phosphorylation sites identified after IMAC enrichment [excel][pdf]
Table S8. Kinase-kinase interactions in the combined KPI, LTP and HTP-HC datasets [excel][pdf]
Table S9. Phosphorylation sites identified in this study [excel][pdf]
Table S10. Yeast strains used in this study [doc][pdf]
Table S11. Plasmids used in this study [doc][pdf]
Table S12. Data used to generate Table S9 phosphorylation sites [excel][pdf]
Table S13. List of probable contaminants removed by SAINT [excel][pdf]
Table S14. Additional proteins removed from final dataset [excel][pdf]
Table S15. Compilation of GO Slim terms [excel][pdf]

Supporting Online Material (Right-Click and Choose 'Save As' to Download Files)

Website Supplementary Table 1 - SAINT Datasets [zip format]

Listing of SAINT interaction data broken into separate files. This archive contains two folders, RAW and FILTERED. RAW contains all the SAINT interactions grouped by TAG with attributes in individual columns delimited by |. FILTERED follows the same format, but only contains interactions with a probability score greater than or equal to 0.85.

Inside the RAW folder, there are four individual files: Inside the FILTERED folder, there are four individual files:

Website Supplementary Table 2 - GO SLIM Process Interactor Overlap [excel format]

GO Slim Process Annotation mappings off all Kinase Associated, Kinase Catalytic, Phosphatase Associated, and Phosphatase Catalytic protein interactors in Our Study, LTP, and HTP-HC.

Description of Columns:

Website Supplementary Table 3 - LTP Dataset [txt format]

All protein interactions from publications curated in the BioGRID that contain less than 100 protein interactions. Excluded from this set are interactions with the Co-Localization, Affinity Capture-RNA, Protein RNA, and Co-Fractionation experimental systems.

Website Supplementary Table 4 - HTP-HC Dataset [txt format]

Start with all protein interactions in the BioGRID from publications containing 100 or more protein interactions, excluding Gavin AC (2002) 11805826, Grandi (2000) 12150911, and Collins (2007) 17200106. Also, interactions with experimental systems of Co-Localization, Affinity Capture-RNA, Protein RNA, and Co-Fractionation are excluded. Each interaction in the initial dataset is checked for multi-validation. Multi-validation occurs if any of the following criteria is met for a given interaction X with interactors A-B. All multi-validated interactions are combined to create HTP-HC.
  1. X is found within the dataset, in reverse order. (ie. A-B is validated by B-A).
  2. X is found within the dataset, with the same A-B, but with a different experimental system.
  3. X is found within the dataset, with the same A-B, but with a different publication.