Welcome to the Yeast Kinase and Phosphatase Interactome (KPI) Resource

The interactions of protein kinases and phosphatases with their regulatory subunits and substrates underpin cellular regulation. We identified a kinase and phosphatase interaction (KPI) network of 1,844 interactions in budding yeast by mass spectrometric analysis of protein complexes. The KPI network contained many dense local regions of interactions that suggested new functions. Notably, the cell cycle phosphatase Cdc14 associated with multiple kinases that revealed roles for Cdc14 in mitogen-activated protein kinase signaling, the DNA damage response and metabolism, while interactions of the target of rapamycin complex 1 (TORC1) uncovered new effector kinases in nitrogen and carbon metabolism. An extensive backbone of kinase-kinase interactions cross-connects the proteome and may serve to coordinate diverse cellular responses.

129 Kinases (click for more details)

29 Kinase Associated (click for more details)

24 Kinase Metabolic/Lipid (click for more details)

30 Phosphatases (click for more details)


7 Phosphatase Associated (click for more details)

5 Phosphatase Metabolic/Lipid (click for more details)

36 Others (click for more details)